A Big MOOve--The Cow Genome is Sequenced

The long sought after cow genome has been sequenced. Heralded as a milestone in animal genetics, unraveling the cow genome will provide scientists with “tantalizing clues to explain the essence of bovinity.” Two papers describing the results of the project will appear in today's issue of the journal Science. 

The cow who donated its DNA for sequencing was a Hereford named LI Dominette 01449 and is one of the estimated 94 million bovines in the US. The project, led by researchers at National Institutes of Health and the U.S. Department of Agriculture, was a gargantuan effort that spanned six years and involved more than 300 scientists from 25 countries and cost only $53 million. Based on sequence analyses, cows haver 22,000 genes as compared with 20,000 to 25,000 for humans. Some of the other findings include: 

  • Cattle and humans have about 80 percent of their genes in common

  • The organization of human chromosomes is closer to that of domestic cattle than to those of rats or mice, which are often used in lab tests of drugs intended for people.

  • Cattle chromosomes, like those of humans and other mammals, contain segmental duplications, which are large, almost identical copies of DNA present in at least two locations in a genome.

  • In domestic cattle, there are duplications related to immunity, metabolism, digestion, reproduction and lactation. Such duplications in humans have been related to a variety of disorders.

Researchers hope that elucidating the bovine genome will help them find ways to improve milk and meat production, develop new strategies to treat and prevent diseases and to reduce the carbon foot print of cows that release large amounts of greenhouse gases that contribute to global warming.

Great news to receive on National DNA Day! 

Science rocks.

Until next time...

Good Luck and Good Job Hunting!!!!!

 

Methicillin Resistant Staphylococcus aureus: A Growing Link between MRSA Infections and Pigs

That methicillin resistant Staphylococcus aureus (MRSA) is in the news again is not surprising. However, to my knowledge, Nicholas Kristof‘s article in today’s New York Times may be the first Op-Ed piece written by a non-scientist about the growing threat and seriousness of MRSA infections. Mr. Kristof apparently became aware of MRSA when he was contacted by Tom Anderson, MD, a Camden, Indiana physician who was experiencing “phenomenal levels of MRSA infections" in his community.

Beginning in the early 1990s, Dr Anderson noticed a rapidly rising incidence in the number of community acquired skin infections caused by MRSA among his patients. Most of Dr Anderson’s patients were swine farmers—the predominant industry in Camden. At first puzzled by the growing incidences of MRSA outbreaks, Dr. Anderson began to suspect that his patient’s pigs may be the source of growing number of cases of MRSA skin infections. He was reluctant to alert public health officials about his suspicions because any hint livestock-related health issues might jeopardize the livelihood of many of his neighbors and friends. By last fall, however, Camden’s MRSA epidemic had grown so large that Dr. Anderson could no longer remain silent. Rather than alert the authorities himself, he decided to invite Mr. Kristof, an investigative reporter, to visit him in Camden and break the story. Unfortunately, before Mr. Kristof could visit, Dr. Anderson died abruptly at age 54. There was no autopsy, but a blood test suggested he may have died from a heart attack or aneurysm. And—this is where the story gets interesting—a recent Dutch study has linked porcine MRSA isolates to a case of human endocarditis. Dr. Anderson had himself suffered at least three bouts of MRSA infections.

In another Dutch study conducted in 2004, MRSA strain ST398 (which caused the endocarditis in the more recent study) was isolated from three family members, three farm workers and 8 of 10 pigs from a single farm. Since then, strain ST398 has spread rapidly through the Netherlands — especially in swine-producing areas— and pig farmers there are 760 times more likely than the general population to carry MRSA. More recently, a study conducted by public health officials in Ontario, Canada showed that 20% of pig farmers were colonized by strains of MRSA genetically identical to those isolated from European pigs. Finally, a 2008 study conducted in Iowa, reported that strain ST398 was isolated from 45 percent of pig farmers and 49 percent of hogs that were tested. Together, these studies suggest that colonization of swine by MRSA and pig farmers is very common and that swine (and possibly other agricultural animals) could become an important reservoir for strains of MRSA.

While not conclusive, most infectious diseases experts believe that the emergence of MRSA and antibiotic resistant bacteria can be directly linked to the widespread and rampant use of antibiotics as growth enhancers in livestock feed. Despite the alarming emergence of multiple antibiotic resistance bacteria, livestock producers in the US and elsewhere continue to add antibiotics to livestock feeds. This led Mr. Kristof to lament that “we as a nation have moved to a model of agriculture that produces cheap bacon but risks the health of all of us.” Not surprisingly, as is frequently the case, big business has chosen to place profits before the health and safety of society.

Until next time...

 

Good Luck and Good Reading (look for Mr. Kristof’s Sunday column on the emergence of antibiotic resistant "superbugs")

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How to Become a Pharmaceutical Scientist

Last week, I had the great fortune to meet (on the Recruiting Animal Show) a career development expert who despite her young age, has seminal insights into today’s job market and how to break into it. Alexandra Levit, a self-avowed GenXer on the GenY cusp, is the author of several books, including They Don't Teach Corporate in College, How'd You Score That Gig? and Success for Hire.

In addition to her book writing activities, Alex’s career advice posts are featured monthly in the Huffington Post and have been showcased in more than 800 media outlets including ABC News, the Associated Press, USA Today, the Wall Street Journal, the New York Times, National Public Radio, Fortune, Yahoo!, and MSN. She is recognized as one of her generations (X or Y?) preeminent career experts who regularly speak at universities and corporations on workplace issues facing young employees.

Alex and I got to talking after Animal' show and I learned that like me, she dabbles in the pharmaceutical career development arena. That said, she graciously agreed to allow me to post a piece that she wrote entitled “Want to Become a Pharmaceutical Scientist? Here’s How!” (see below) that offers guidance for entry level scientist who are considering careers in the pharmaceutical or biotechnology industries. Read and learn!

"Want to Become a Pharmaceutical Scientist? Here’s How!"

Talk about a career with intriguing possibilities. People entering the pharmaceutical research field today may be the ones discovering the cure for cancer or Parkinson’s disease tomorrow. Although the United States’ pharmaceutical companies sometimes get a bad rap, they routinely make astounding advances that save lives, and from what I hear, aren’t bad places to work either. If you’re a data-head who wants to use your scientific background for the greater good, you might consider becoming a pharmaceutical scientist. Here’s some detail about what breaking into the field entails.

According to the American Association of Pharmaceutical Scientists, the pharmaceutical sciences combine a broad range of scientific disciplines that are critical to the discovery and development of new drugs, therapies, and medical devices. Some of the more common specializations include drug discovery and design, whichdeals with the design and synthesis of new drug molecules and includes medicinal chemistry, combinatorial chemistry, and biotechnology; drug delivery, which is concerned with the design of dosage forms – such as tablets, injections or patches – that deliver the drug to the site of action within a patient; drug action, which examines how the drug works in a living system; clinical pharmacology, which is concerned with the use of drugs in the treatment of diseases and leverages human clinical trials to determine efficacy, adverse effects, and drug-to-drug interaction; and drug analysis, which involves separating, identifying, and quantifying the components of a sample.

Twenty-eight year old Matt, a senior research associate at Novartis, spends his days engaged in both drug action and drug analysis for the company’s wide range of prescription medications for ailments such as heart disease, high blood pressure, and diabetes. He’s responsible for testing animal organ and plasma samples to determine if a drug is working the way it’s supposed to. “It’s my job to quantify the drug’s efficacy in the body, to gather early support as it moves through the process of getting approved by the Food and Drug Administration,” he says. 

While the majority of pharmaceutical scientists are employed by private-sector pharmaceutical companies, others work as researchers and professors at universities, as regulatory scientists for government agencies like the Food and Drug Administration (FDA), or as researchers at national laboratories such as the National Institutes of Health (NIH). An undergraduate degree in chemistry, biology, pharmacy, or engineering is a prerequisite for the entry-level position of research assistant. The research assistant, who receives a broad-based introduction to the pharmaceutical world, records, stores and summarizes information and data, prepares technical reports, and develops laboratory skills and familiarity with equipment. “You should expect that your first job will be low level and the work very routine,” says Matt. “You won’t have much of a chance to be creative or innovative, and you have to be tolerant of that at the beginning of your career.” Adds Beth, 31, an R&D project director at a top pharmaceutical company in the Northeast: “Make the most of each opportunity, no matter how small. Success with smaller projects will directly lead to greater responsibility and rapid professional growth. Focus both on delivering results and on how you deliver the results: for example, through strong teamwork or a rigorous research approach.”

Recent college grads looking for research assistant jobs may want to start by educating themselves about the pharmaceutical industry in general. The Pharmaceutical Research and Manufacturers of America Web site (www.phrma.org) and the American Association of Pharmaceutical Scientists Web site (www.aapspharmaceutica.com) are great places to check out relevant publications, networking events, and internship and employment listings. Specific job opportunities can also be found on industry job portals including www.biospace.com, www.hirerx.com, and www.medzilla.com, and you might also take advantage of openings advertised through your college’s career center. “My first paying job was an internship at a medical device company,” says Beth. “I made the original contact at an MIT career fair and then interviewed on campus with the company’s recruiting team. I shared my passion for medical device engineering and my solid academic background and was hired after my sophomore year.”

Matt also suggests that you might get in the door by connecting with a temporary employment firm specializing in pharmaceuticals. “I signed on with Kelly Scientific right out of school,” he says. “Almost immediately, I got contract work doing clinical research studies that eventually resulted in a full-time job.” Women may find that they’re especially marketable, as the pharmaceutical industry is still predominantly male and most organizations are striving to even things out.

The typical pharmaceutical research career has ample room for growth. From the position of research assistant, you can progress to a research associate, an associate scientist, a scientist, a senior scientist, and a principal scientist. After you’ve reached a certain level, you may also have the option of taking on a cross-functional role in research management, regulatory affairs, pharmacoeconomics, or communications. At the moment, most people who reach the top levels of the profession get a Ph.D. along the way, but many say that’s changing and that experience in the lab is increasingly worth more than formal education. You’ll be happy to learn that compared to other industries, compensation is quite generous. According to a recent American Association for the Advancement of Science Salary Survey, pharmaceutical research assistants make more than $65K with just a bachelor’s degree, and six-figure incomes are standard after a few years.

Like other data-heads, pharmaceutical scientists are highly analytical and have had the math and science coursework to back up their natural talent. “You have to be anal about the details, because one small error can mess up a whole batch of samples,” says Matt. A cool-headed approach to troubleshooting is critical as well. “Development setbacks occur frequently,” says Beth. “When something goes wrong, my first step is to pull the team together to diagnose the situation. I do not try to lay blame but want to ensure that we learn and do not repeat the mistake or ignore the issue as we move forward.” Additionally, pharmaceutical research environments can be political and difficult to navigate at times. “Drug research isn’t always run like a well-oiled machine and loyalty to the company doesn’t always work in your favor,” says Matt. “You have to have a good sense of what you’re worth and what you have to offer.”

If you think pharmaceutical research might be for you, now’s a great time to test drive it as a career. A special supplement on hiring trends in the pharmaceutical space published in The Scientist in 2005 reported that the number of people employed in the U.S. industry is expected to grow from 413,700 to 536,000 in the next decade, and that Research & Development spending, which exploded in 2004, is still steadily increasing. “I love my job because I have the opportunity to impact patients with my daily work, collaborating with my fellow team members to solve complex problems,” says Beth. “And I feel very fortunate to work at a company where my personal values align very closely with the corporate culture.”

Until next time…

Good Luck and Good Job Hunting!!!!!!

New Jersey Dog Breeder Darkside: Redux

Since the original post about bogus dog breeding in New Jersey, I received several comments from the alleged breeder(s)" who sold us our dogs Moose and Sandy.  I invite you to read them---they are very interesting to say to least. 

A person who became involved with our little doggy drama sent me a link to a company that does genetic testing verify or refute canine breed or pedigree claims.  We are going to have both of our dogs tested.  We suspect that Moose is not a havanese as billed but a maltese/bichon or maybe a maltese/havanese mix . As luck would have it, we met both a maltese and bichon during our trip to Moosehead Lake in Maine and learned alot about both breeds. Moose looks alot like a maltese but unlike pure malteses who are all white, he has quite a bit of cream coloring in his coat.

The jury is still out on Sandy (who we bought as a 10 week old puppy) is suppose to be a full-blooded havanese female.  She looks like havanese that we have seen in photos, but given the circumstances we are not certain that she is really what she is supposed to be!

If either or both of the dogs are not what they were claimed to be in the pedigree papers that we received, it is our belief that we have a good case of fraud or at the very least misrepresentation on our hands.  As most of us know, DNA doesn't lie!  Who says that Cary Mullis didn't deserve to win the Nobel Prize for developing PCR!!!!!!!!!

Please read the back story (see below) about our journey into the underbelly of bogus dog breeding in the Garden State!!!

Original Post

Several months ago, my wife and daughter saw a “really adorable” dog at a school fundraising event.  My wife asked the owner what type of dog it was and she was told “it is a Havanese.” Neither my wife nor I had heard of the breed before but, after doing some Internet research we learned that it is related to the Bichon and is called Havanese because the breed originated in Cuba. As former residents of Miami (we met there) and familiar with all things Cuban my wife and I decided to buy a Havanese puppy for our daughter and two sons (they had been vigorously campaigning for a dog for the past few years).

The first step in our search was to Google the breed and see whether there were any Havanese breeders in New Jersey. Much to our delight, we found five Havanese breeders in New Jersey and no fewer than three were in driving distance from our home. To make a long story short, we purchased a wonderful 10-week old Havanese puppy called Sandy from a private breeder named Grace Abrams who runs Havanese 4Ever in Vincentown, NJ. A couple of days after we brought Sandy home, my wife took her to her first veterinary appointment and disappointed to learn that Sandy had serious case of ear mites and a round worm infestation. While we concerned, we didn’t think much of this (although Grace had certified that the puppy was healthy and disease free) and went on with our newly-minted Havanese existence.

After living with Sandy for several months (and spending way too much money to build a fence for one dog), my wife and I decided that Sandy needed a companion to play with. Imagine our delight, when we saw that Grace was looking for a home for a young, Havanese male that she had recently acquired “from a couple relocating from North Carolina to New York City.”  After seeing the ad on Grace’s website, my wife, Sandy and I drove back to Vincentown examine the “9 month old healthy Havanese male” that was dumped on Grace’s door step. Grace informed us that she had been caring for the dog for two weeks and that he was filthy and in very bad condition when she first got him. Without any hesitation, we adopted the dog (Moose) for $400 (after Grace knocked off $150 because of the ear mites and worms with Sandy) and thought all was right with the world. We also received some nominal paperwork including Moose’s veterinary records, his pedigree papers and a receipt.

A couple of days after we brought Moose home, my wife took him to the vet for a physical examination. Much to her dismay, the veterinarian told my wife that Moose was not 9 months old but closer to 3 years, had a bad case of gingivitis (and 4 loose teeth that needed to be pulled) and an ear and urinary tract infections. After receiving three frantic phone calls from my wife who was in tears, I contacted Grace and informed her that things were not right with Moose and that we wanted our money back. Again, to make a long story short, Grace refused to give us a refund unless we brought Moose back to her. Of course, we didn’t comply and Moose (who is a wonderful, loyal and sweet dog) is now an official member of the family. That said, with the help of a pet advocacy organization, NJCAPSA which has been investigating puppy mill fraud in NJ, we learned that Grace Abrams is a member of a notorious ring of South Jersey dog grifters run by her mother Donna Roberts who in 2007 was found guilty under NJ Statute 4:22-26L on three of five counts of animal cruelty.

Grace aka Dawn Abrams and her mother Donna Roberts purportedly purchases puppies from Pennsylvania Amish and Mennonite puppy mills (allegedly run by a nefarious character named David Zimmerman) and sell them from Boston to Washington DC as well as locally in New Jersey. Although never convicted of fraud, numerous complaints have been filed against Donna and Grace claiming that they supplied customers with falsified veterinary certificates and bogus AKC or other registration papers. Sometimes, Robert Cohen, an odious licensed New Jersey veterinarian, issues “health certificates” for Donna/Dawn’s puppies and so-called “rescue animals”. Not surprisingly, Cohen’s name appeared in court documents in several cases of fraud brought against Donna Roberts.

Because of her convictions, Donna Roberts keeps a low profile. Grace/Dawn Abrams is the front person but her mother Donna Roberts actually calls all the shots. Over the years, Donna and Grace/Dawn have sold dogs under the following kennel names: 1) Dark Moon Havanese, Shamong, NJ,  2) Shady Oaks Havanese, Howell NJ, 3) Kissy Face Havanese, Lakewood NJ, 4) Havanese Breeze, Medford NJ, 4) Havanese 4U, Indian Mills, NJ and 5) Havanese 4ever, Vincentown, NJ. Be warned that they also advertise Havanese and other breeds on PetFinder, Craig’s List, Breeders.net, Doggie.com and others pet sites where there are no ad listing charges.

Also, be aware that they are alleged to sell dogs under the following names or aliases:

Dee Woods

Dee Roberts

Gloria Anderson

Donna Anderson

Rachel Anderson

Donna Marie Dueben

Donna Truex

Robin Lewis

Robin Anderson (Donna Robert’s sister)

Marci Jean - advertises under the same phone number as Shady Oak

Carol Lang

Grace Abrams - Havanese4ever

Paul - Shady Oak Havanese

Marci Dueben - Havana Breeze Havanese

After we learned about Donna and her co-conspirators, my wife and I (who is an attorney) thoroughly examined the papers that we received for Sandy and Moose from Grace Abrams. Sure enough, no fewer than five of their aliases appeared in various capacities (e.g., owner, seller, breeder etc) throughout our documents. Further, Robert Cohen was the veterinarian of record who signed all of Sandy’s veterinary certificates. Although Moose was billed as a 9 month Havanese we now believe that he is a 2 year old Bichon, Shih Tzu or a mix. We think that he was probably a former breeder dog in a PA puppy mill (since he was not neutered). Sandy is likely a true Havanese but I wouldn’t be surprised that turns out not to be the case.  

I wrote this piece because the emotional and gut-wrenching experience that my family and I have gone through has had a profound effect on me. To that end, I am going to do everything possibly to shut down the operations run by Donna Roberts, Grace/Dawn Abrams and their co-conspirators.  Any suggestions, ideas, help (especially Internet/technology-wise) on how to put these people and their ilk out of business would be greatly appreciated.  Please feel free to repost this piece on any appropriate pet and dog breeder sites or blogs.

Until next time….

Good Luck and Good Puppy Hunting!!!!

BioJobBlog Talks with the Recruiting Animal

I was contacted by the Recruiting Animal to see whether I would be interested in joining him on his show, to talk about recruiting in the pharmaceutical and biotechnology fields. Although I am a recovering recruiter and have not placed a single candidate in the past 5 years, I decided what the heck—what do I have to lose?

For those of you who are interested you can hear my pearls of wisdom by clicking the icon below.

 

 

Until next time…

Good Luck and Good Job Hunting!!!!!!